产品基本信息

• 产品货号 RM9249
• 别名 FEN1;RAD2;Flap endonuclease 1;FEN-1;DNase IV;Flap structure-specific endonuclease 1;Maturation factor 1;MF1;hFEN-1
• 产品名称 FEN-1 (2F10) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 FEN1
• 蛋白名称 Flap endonuclease 1
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 2237
• Human Swissprot No. P39748
• Mouse Swissprot No. P39749
• Rat Gene ID 84490
• Rat Swissprot No. Q5XIP6
• 特异性 Endogenous
• 稀释度 IHC-P 1:500-1:2000, WB 1:2000-1:10000, IF 1:200-1:1000, ELISA 1:5000-1:20000
• 参考分子量 50kDa
• 预测分子量 43kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 IgG,Kappa
• 背景介绍 The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
• 细胞定位 [Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .

图片

产品详情