产品基本信息

• 产品货号 B1029
• 产品名称 Actin beta (5B7) Mouse Monoclonal Antibody
• 类别 抗体产品
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P,Elisa
• 反应种属 Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS,pH 7.4, containing 50% glycerol，2% BSA and New type preservative N.
• 免疫原 Synthetic Peptide of β-actin
• 特异性 The antibody detects endogenous β-actin protein.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:5000, IHC-P 1:200
• 参考分子量 42KD
• 宿主 Mouse
• 纯化 The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

图片

	Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
	Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: AGS Lane 2: A375 Lane 3: A431 Lane 4: A549 Lane 5: ACHN Lane 6: Daudi Lane 7: Du145 Lane 8: H69AR Lane 9: HACAT Lane 10: HepG2
	Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HUVEC Lane 2:JAR Lane 3:Jurkat Lane 4:LN18 Lane 5:MCF7 Lane 6:MES-SA Lane 7:MG-63 Lane 8:MRC-5 Lane 9:NIH-3T3 Lane 10:OVCAR-3
	Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-3 Lane 2: PC-12 Lane 3: Raji Lane 4: Ramos Lane 5: Saos-2 Lane 6: RAW264.7 Lane 7: SH-SY5Y Lane 8: SW480 Lane 9: T47D Lane 10: U2OS
	Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: Karpas-299 Lane 3: Hela Lane 4: HEK293 Lane 5: Mouse Lung Lane 6: mouse testis Lane 7: Rabbit brain

产品详情