产品基本信息

• 产品货号 BD-PK0013
• 别名 HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK;H4K12AC;HIST1H4B;HIST1H4C;HIST1H4D;HIST1H4E;HIST1H4F;HIST1H4H;HIST1H4I;HIST1H4J;HIST1H4K;HIST1H4L;HIST2H4A;HIST2H4B;HIST4H4;
• 产品名称 Histone H4 (Acetyl Lys12) Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 HIST1H4A
• 蛋白名称 Histone H4
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat,Monkey
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=121504
• Human Swissprot No. P62805
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P62805/entry
• Mouse Gene ID 1.00041e+008
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100041230
• Mouse Swissprot No. P62806
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P62806
• Rat Gene ID 1.00361e+008
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100360950
• Rat Swissprot No. P62804
• Rat Swissprot Link http://www.uniprot.org/uniprot/P62804
• 免疫原 The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12. AA range:10-59
• 特异性 Acetyl-Histone H4 (K12) Polyclonal Antibody detects endogenous levels of Histone H4 protein only when acetylated at K12.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:10000.Not yet tested in other applications.
• 预测分子量 11kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],
• 组织表达 B-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call
• 细胞定位 Nucleus. Chromosome.
• 信号通路 Protein_Acetylation
• 功能 function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.,PTM:Sumoylated, which is associated with transcriptional repression.,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.,similarity:Belongs to the histone H4 family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of HeLa cells, using Histone H4 （Acetyl-Lys12） Antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 （Acetyl-Lys12） Antibody. The picture on the right is blocked with the synthesized peptide.
	Western Blot analysis of 293 cells using Acetyl-Histone H4 （K12） Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000
	Western blot analysis of Mouse-kidney HELA lysis using Acetyl-Histone H4 （K12） antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
	Western Blot analysis
	Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys12) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

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