产品基本信息

• 产品货号 BD-PM3046
• 别名 PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45
• 产品名称 CD45(12A9)Mouse Monoclonal Antibody
• 类别 抗体产品
• 基因名称 PTPRC
• 蛋白名称 Receptor-type tyrosine-protein phosphatase C
• Clonality Monoclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P
• 反应种属 Human
• 存储缓冲液 PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
• Human Gene ID 5788
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5788
• Human Swissprot No. P08575
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P08575/entry
• Mouse Swissprot No. P06800
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P06800
• Rat Gene ID 24699
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24699
• Rat Swissprot No. P04157
• Rat Swissprot Link http://www.uniprot.org/uniprot/P04157
• 免疫原 Synthetic Peptide of CD45
• 特异性 The antibody detects endogenous CD45 proteins.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:2000, IHC-P 1:50-300
• 预测分子量 147kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Mouse
• 背景介绍 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which enc
• 组织表达 Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
• 细胞定位 Cell membrane ; Single-pass type I membrane protein . Membrane raft . Colocalized with DPP4 in membrane rafts. .
• 信号通路 Cell adhesion molecules (CAMs);T_Cell_Receptor;Fc gamma R-mediated phagocytosis;Primary immunodeficiency;
• 功能 alternative products:At least 8 isoforms are produced,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,disease:Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.,disease:Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.,domain:The first PTPase domain interacts with SKAP1.,function:Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.,online information:CD45 entry,online information:PTPRC mutation db,PTM:Heavily N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.,similarity:Contains 2 fibronectin type-III domains.,similarity:Contains 2 tyrosine-protein phosphatase domains.,subunit:Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.,
• 纯化 The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

图片

	Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody（12A9）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody（12A9） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western blot analysis of Hela, diluted at 1:2000.

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