产品基本信息

• 产品货号 BD-PM3687
• 别名 PDGFRA
• 产品名称 PDGFRα(7A3)Mouse Monoclonal Antibody
• 类别 抗体产品
• 基因名称 PDGFRA
• 蛋白名称 PDGFRA
• Clonality Monoclonal
• 推荐应用 IF-P,IF-F,IF-ICC,IHC-P
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 5156
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5156
• Human Swissprot No. P16234
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P16234/entry
• Mouse Swissprot No. P26618
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P26618
• Rat Swissprot No. P20786
• Rat Swissprot Link http://www.uniprot.org/uniprot/O54941P20786
• 免疫原 Synthetic Peptide of PDGFRα at AA range of 1010-1090
• 特异性 PDGFRα protein detects endogenous levels of PDGFRA
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, IHC-P 1:100-200
• 预测分子量 180kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Mouse
• 背景介绍 This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012],
• 组织表达 Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
• 细胞定位 Cell membrane ; Single-pass type I membrane protein . Cell projection, cilium . Golgi apparatus .
• 信号通路 MAPK_ERK_Growth;MAPK_G_Protein;Calcium;Cytokine-cytokine receptor interaction;Endocytosis;Focal adhesion;Gap junction;Regulates Actin and Cytoskeleton;Pathways in cancer;Colorectal cancer;Glioma;Prostate cancer;Melanoma;
• 功能 catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.,function:Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Homodimer, and heterodimer with PDGFRB. Interacts with the SH2 domain of SHB via phosphorylated Tyr-720 (By similarity). Interacts with the SH2 domain of SHF via phosphorylated Tyr-720.,tissue specificity:Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.,
• 纯化 The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

图片

	Immunofluorescence analysis of mouse-spleen tissue. 1,PDGFRα Mouse Monoclonal Antibody（7A3）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,PDGFRα Mouse Monoclonal Antibody（7A3） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

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