产品基本信息

• 产品货号 BD-PP0114
• 别名 FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
• 产品名称 FoxO1 (phospho Ser319) Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 FOXO1
• 蛋白名称 Forkhead box protein O1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 2308
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2308
• Human Swissprot No. Q12778
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q12778/entry
• Mouse Gene ID 56458
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56458
• Mouse Swissprot No. Q9R1E0
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9R1E0
• Rat Gene ID 84482
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84482
• Rat Swissprot No. G3V7R4
• Rat Swissprot Link http://www.uniprot.org/uniprot/G3V7R4
• 免疫原 The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser319. AA range:286-335
• 特异性 Phospho-FoxO1 (S319) Polyclonal Antibody detects endogenous levels of FoxO1 protein only when phosphorylated at S319.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 82kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
• 组织表达 Ubiquitous.
• 细胞定位 Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527). Phosphorylation by NLK results in nuclear export (By similarity). Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179, PubMed:21245099). SGK1-mediated phosphorylation also results in nuclear translocation (By similarity). Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide (By similarity). Retained in the nucleus on methylation (By similarity). PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815). Deacetylation by SIRT6, promotes its translocation into the cytoplasm (PubMed:25009184). .
• 信号通路 Insulin Receptor; B Cell Receptor; Protein_Acetylation
• 功能 disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Interacts with LRPPRC.,tissue specificity:Ubiquitous.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using FKHR （Phospho-Ser319） Antibody
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR （Phospho-Ser319） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from HeLa cells treated with EGF, using FKHR （Phospho-Ser319） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of various cells using Phospho-FoxO1 （S319） Polyclonal Antibody
	Western Blot analysis of 293 cells using Phospho-FoxO1 （S319） Polyclonal Antibody

产品详情