产品基本信息

• 产品货号 BD-PP1230
• 别名 Death-associated protein kinase 1 (DAP kinase 1;EC 2.7.11.1);DAPK
• 产品名称 DAPK1 (Phospho Ser308) Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 DAPK1
• 蛋白名称 DAPK1 (Phospho-Ser308)
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC,WB
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1612
• Human Swissprot No. P53355
• Human Swissprot Link https://www.uniprot.org/uniprotkb/P53355/entry
• 免疫原 Synthesized peptide derived from human DAPK1 (Phospho-Ser308)
• 特异性 This antibody detects endogenous phospho levels of DAPK1 (Phospho-Ser308) at Human:S308, Mouse:S308
• 稀释度 IHC-P 1:50-200, WB 1:500-2000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 160kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
• 组织表达 Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
• 细胞定位 [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,
• 纯化 The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

图片

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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