MED28 (13O16) Mouse Monoclonal antibody

货号: BD-PE4708
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规格 价格 货期 数量
  • 50μl
    1200.00
    1个工作日
    100μl
    2200.00
    1个工作日

产品基本信息

  • 产品货号 BD-PE4708
  • Uniprot ID Q9H204
  • Gene ID NM_025205
  • 产品名称 MED28 (13O16) Mouse Monoclonal antibody
  • 类别 抗体产品
  • 基因名称 MED28
  • Clonality Monoclonal
  • 推荐应用 IHC-P,IHC-F,WB
  • 反应种属 Human,Mouse,Rat
  • 存储缓冲液 PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
  • 免疫原 Full length human recombinant protein of human MED28 (NP_079481) produced in E.coli.
  • 稀释度 WB 1:500-2000, IHC-P/IHC-F 1:150
  • 预测分子量 19.3kDa
  • 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
  • 宿主 Mouse
  • 同种型 IgG2b
  • 纯化 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

图片

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MED28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4708) (1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MED28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4708) (1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED28 (Cat# BD-PE4708)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.
Western blot analysis of extracts (35ug) from 293T cell line by using anti-MED28 monoclonal antibody (1:500).

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