产品基本信息

• 产品货号 B2208
• Uniprot ID P68431
• Gene ID 8350
• 别名 H3/A; H3FA
• 产品名称 Histone H3 (14J7) Rabbit Monoclonal Antibody
• 类别 抗体产品
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-ICC,FCM
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
• 免疫原 A synthetic peptide of human Histone H3
• 稀释度 WB 1:1000, IHC-P/IF-P 1:2000-1:10000, IF-ICC 1:500-1:2000, FCM 1:50
• 参考分子量 17kDa
• 预测分子量 15kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 IgG
• 背景介绍 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
• 纯化 Affinity Purification

图片

	Western blot analysis of extracts from Hela，3T3 cells using B2208 at 1:1000.
	Immunofluorescence analysis of A431 cells labelling Histone H3 with B2208. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with B2208 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 Control: Secondary antibody only.
	Western blot analysis of extracts from Mouse teatis tissue using B2208 at 1:50000.

产品详情