产品基本信息

• 产品货号 RM8262
• 别名 KLF4;EZF;GKLF;Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
• 产品名称 KLF4 (6W15) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 KLF4
• 蛋白名称 Krueppel-like factor 4
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 9314
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9314
• Human Swissprot No. O43474
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O43474/entry
• Mouse Swissprot No. Q60793
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q60793
• 稀释度 IHC-P 1:200-1000,WB 1:1000-5000,IF-P/IF-F/IF-ICC 1:200-1000,ELISA 1:5000-20000,IP 1:50-200
• 参考分子量 60kDa
• 预测分子量 55kDa
• 运输及保存条件 -20°C/1 year(Do not lower than -25°C)
• 宿主 Rabbit
• 同种型 Monoclonal, rabbit, IgG, Kappa
• 背景介绍 This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015],
• 功能 function:Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interaction with the C-terminal domain of MUC1 enhances suppression of TP53/p53 transcription.,
• 纯化 Protein A

图片

	Western blot analysis of lysates from HCT-116 cells, using KLF4 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
	Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, primary Antibody was diluted at 1:200(4℃,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
	Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, primary Antibody was diluted at 1:200(4℃,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
	Immunohistochemical analysis of paraffin-embedded rat colon tissue. 1, primary Antibody was diluted at 1:200(4℃,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

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