产品基本信息

• 产品货号 BD-PT2912
• 别名 MMP16; MMPX2; Matrix metalloproteinase-16; MMP-16; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP
• 产品名称 MMP-16 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 MMP16
• 蛋白名称 Matrix metalloproteinase-16
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 4325
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4325
• Human Swissprot No. P51512
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P51512/entry
• Mouse Gene ID 17389
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17389
• Mouse Swissprot No. Q9WTR0
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9WTR0
• Rat Gene ID 65205
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=65205
• Rat Swissprot No. O35548
• Rat Swissprot Link http://www.uniprot.org/uniprot/O35548
• 免疫原 The antiserum was produced against synthesized peptide derived from human MMP-16. AA range:551-600
• 特异性 MMP-16 Polyclonal Antibody detects endogenous levels of MMP-16 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 70kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010],
• 组织表达 Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
• 细胞定位 [Isoform Long]: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells.; [Isoform Short]: Secreted, extracellular space, extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
• 信号通路 Angiogenesis
• 功能 cofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,developmental stage:Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:TIMP-2 shows little inhibitory activity compared to TIMP-1. TIMP-1 seems to have less binding affinity than TIMP-2 for the short isoform.,function:Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. The short isoform cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subcellular location:Localized at the cell surface of melanoma cells.,subunit:Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.,tissue specificity:Expressed in heart, brain, placenta, ovary and small intestine. The short isoform is found in the ovary.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MMP-16 Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from HepG2 cells, using MMP-16 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using MMP-16 Polyclonal Antibody diluted at 1：500

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