产品基本信息

• 产品货号 RM8523
• 别名 CANX;Calnexin;IP90;Major histocompatibility complex class I antigen-binding protein p88;p90
• 产品名称 Calnexin (8A6) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 CANX
• 蛋白名称 Calnexin
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 821
• Human Swissprot No. P27824
• Mouse Gene ID 12330
• Mouse Swissprot No. P35564
• Rat Gene ID 29144
• Rat Swissprot No. P35565
• 特异性 Endogenous
• 稀释度 IHC-P 1:2000-1:10000, WB 1:1000-1:5000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000
• 参考分子量 90kDa
• 预测分子量 68kDa
• 运输及保存条件 -20°C/1 year(Do not lower than -25°C)
• 宿主 Rabbit
• 同种型 IgG,Kappa
• 背景介绍 This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008],
• 组织表达 B-cell lymphoma,Epithelium,Fibroblast,Keratinocyte,Kidney,Liver,Lymph,Placenta,Platelet,
• 细胞定位 Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). .
• 功能 Function:Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.,online information:Calnexin entry,similarity:Belongs to the calreticulin family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
• 纯化 Protein A

图片

THP-1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

产品详情