产品基本信息

• 产品货号 RM8585
• 别名 MYL9;MLC2;MRLC1;MYRL2;Myosin regulatory light polypeptide 9;20 kDa myosin light chain;LC20;MLC-2C;Myosin RLC;Myosin regulatory light chain 2;smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRL
• 产品名称 MYL9 (Phospho Thr18/Ser19) (13O12) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 MYL9
• 蛋白名称 Myosin regulatory light polypeptide 9
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 10398;10627
• Human Swissprot No. P24844
• Mouse Gene ID 98932
• Mouse Swissprot No. Q9CQ19
• Rat Gene ID 296313
• Rat Swissprot No. Q64122
• 特异性 MYL9 (Phospho Thr18/Ser19) Antibody detects endogenous levels of MYL9 protein only when phosphorylated at Thr18/Ser19.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RAtsNV
• 稀释度 IHC-P 1:50-1:400, WB 1:1000-1:5000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000
• 参考分子量 20kDa
• 预测分子量 20kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 IgG,Kappa
• 背景介绍 Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
• 组织表达 Smooth muscle tissues and in some, but not all, nonmuscle cells.
• 细胞定位 Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts. .
• 功能 Function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Smooth muscle tissues and in some, but not all, nonmuscle cells.,
• 纯化 Protein A

图片

Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

产品详情