产品基本信息

• 产品货号 BD-PT3497
• 别名 CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
• 产品名称 p21 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 CDKN1A
• 蛋白名称 Cyclin-dependent kinase inhibitor 1
• Clonality Polyclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1026
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1026
• Human Swissprot No. P38936
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P38936/entry
• Mouse Gene ID 12575
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12575
• Mouse Swissprot No. P39689
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P39689
• 免疫原 The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160
• 特异性 p21 Polyclonal Antibody detects endogenous levels of p21 protein.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:500-1:2000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 21kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac
• 组织表达 Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
• 细胞定位 Cytoplasm . Nucleus .
• 信号通路 Stem cell pathway; ErbB/HER; PI3K/Akt; AMPK; Cell_Cycle_G1S;Cell_Cycle_G2M_DNA; Protein_Acetylation
• 功能 function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

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