产品基本信息

• 产品货号 RM9421
• 别名 VAV1;VAV;Proto-oncogene vav
• 产品名称 VAV1 (5C7) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 VAV1
• 蛋白名称 Proto-oncogene vav
• Clonality Monoclonal
• 推荐应用 WB,IF-P,IF-F,IF-ICC,IP,ELISA
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 7409
• Human Swissprot No. P15498
• Mouse Gene ID 22324
• Mouse Swissprot No. P27870
• Rat Swissprot No. P54100
• 特异性 Endogenous
• 稀释度 WB 1:2000-1:10000;IF-P/IF-F/IF-ICC 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
• 参考分子量 98kDa
• 预测分子量 98kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 IgG,Kappa
• 背景介绍 This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012],
• 组织表达 Widely expressed in hematopoietic cells but not in other cell types.
• 细胞定位 intracellular,cytosol,plasma membrane,cell-cell junction,
• 功能 Domain:The DH domain is involved in interaction with CCPG1.,Function:Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.,miscellaneous:'Vav' stands for the sixth letter of the Hebrew alphabet.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:May interact with CCPG1 (By similarity). Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK.,tissue specificity:Widely expressed in hematopoietic cells but not in other cell types.,

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Western Blot analysis using Jurkat cell lysate, Proteins were separated by 4-20% SDS-PAGE

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