产品基本信息

• 产品货号 RM9427
• 别名 PRKD2;PKD2;HSPC187;Serine/threonine-protein kinase D2;nPKC-D2
• 产品名称 PKD2 (7B13) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 PRKD2
• 蛋白名称 Serine/threonine-protein kinase D2
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 存储缓冲液 PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Human Gene ID 25865
• Human Swissprot No. Q9BZL6
• Mouse Gene ID 101540
• Mouse Swissprot No. Q8BZ03
• Rat Gene ID 292658
• Rat Swissprot No. Q5XIS9
• 特异性 Endogenous
• 稀释度 IHC-P 1:200-1:500;WB 1:2000-1:10000;IF-P/IF-F/IF-ICC 1:200-1:1000;ELISA 1:5000-1:20000;
• 参考分子量 115kDa
• 预测分子量 97kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 IgG,Kappa
• 背景介绍 The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
• 组织表达 Widely expressed.
• 细胞定位 Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .
• 功能 Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,Function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,

图片

Immunohistochemical analysis of paraffin-embedded Human tonsil

产品详情