产品基本信息

• 产品货号 BD-PT5053
• 别名 ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
• 产品名称 α-SMA Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 ACTA2
• 蛋白名称 Actin aortic smooth muscle
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 59
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=59
• Human Swissprot No. P62736
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P62736/entry
• Mouse Gene ID 11475
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11475
• Mouse Swissprot No. P62737
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P62737
• Rat Gene ID 81633
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81633
• Rat Swissprot No. P62738
• Rat Swissprot Link http://www.uniprot.org/uniprot/P62738
• 免疫原 Synthesized peptide derived from the N-terminal region of human α-SMA. AA range: 84-134
• 特异性 α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 42kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],
• 组织表达 Pituitary,Uterus,
• 细胞定位 Cytoplasm, cytoskeleton.
• 信号通路 Vascular smooth muscle contraction;
• 功能 disease:Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the actin family.,subunit:Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

产品详情