产品基本信息

• 产品货号 BD-PT5692
• 别名 EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
• 产品名称 p300 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 EP300
• 蛋白名称 Histone acetyltransferase p300
• Clonality Polyclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 2033
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2033
• Human Swissprot No. Q09472
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q09472/entry
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=
• Mouse Swissprot No. B2RWS6
• Mouse Swissprot Link http://www.uniprot.org/uniprot/B2RWS6
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=
• Rat Swissprot Link http://www.uniprot.org/uniprot/
• 免疫原 Synthesized peptide derived from the Internal region of human p300.
• 特异性 p300 Polyclonal Antibody detects endogenous levels of p300 protein.
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000.Not yet tested in other applications.
• 预测分子量 300kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],
• 组织表达 Epithelium,Skin,
• 细胞定位 Cytoplasm . Nucleus . Chromosome . Localizes to active chromatin: Colocalizes with histone H3 acetylated and/or crotonylated at 'Lys-18' (H3K18ac and H3K18cr, respectively) (PubMed:25818647). In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus (PubMed:12929931). .
• 信号通路 Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;WNT;WNT-T CELLNotch;TGF-beta;Adherens_Junction;Jak_STAT;Long-term potentiation;Melanogenesis;Huntington's disease;Pathways in cancer;Renal cell carcinoma;Prostate cancer;
• 功能 catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,disease:Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3.,disease:Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.,disease:Defects in EP300 may play a role in epithelial cancer.,function:Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.,online information:P300/CBP entry,PTM:Acetylated on Lys at up to 17 positions by intermolecular autocatalysis.,PTM:Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.,PTM:Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.,PTM:Phosphorylated.,similarity:Contains 1 bromo domain.,similarity:Contains 1 KIX domain.,similarity:Contains 1 ZZ-type zinc finger.,similarity:Contains 2 TAZ-type zinc fingers.,subunit:Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, PRDM6, SPIB, SRY, TCF7L2, TP53, SRCAP, TTC5, JMY and TRERF1. The TAZ-type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with ING5. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of rat-kidney tissue. 1,p300 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-kidney tissue. 1,p300 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,p300 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,p300 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,p300 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,p300 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western blot analysis of HELA 293T 823-AV using EP300 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
	Immunofluorescence analysis of Hela cell. 1,p300 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). p53 Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

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