产品基本信息

• 产品货号 BD-PT6055
• 别名 Sterol regulatory element-binding protein 1 (SREBP-1) (Class D basic helix-loop-helix protein 1) (bHLHd1) (Sterol regulatory element-binding transcription factor 1) [Cleaved into: Processed sterol regulatory element-binding protein 1]
• 产品名称 SREBP-1 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 SREBF1 BHLHD1 SREBP1
• 蛋白名称 SREBP-1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat,Golden hamster
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 6720
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6720
• Human Swissprot No. P36956
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P36956/entry
• Mouse Gene ID 20787
• Mouse Swissprot No. Q9WTN3
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q9WTN3
• 免疫原 Synthesized peptide derived from human SREBP-1. at AA range: 250-330
• 特异性 This antibody detects endogenous levels of SREBP-1
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 110kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016],
• 组织表达 Expressed in a wide variety of tissues, most abundant in liver and adrenal gland (PubMed:8402897). In fetal tissues lung and liver shows highest expression (PubMed:8402897). ; [Isoform SREBP-1A]: Predominates in hepatoma cell lines (PubMed:8402897). Also expressed in kidney, brain, white fat, and muscle (PubMed:8402897). ; [Isoform SREBP-1C]: Predominantly expressed in liver and adipose tissues (PubMed:8402897). Also expressed in kidney, brain, white fat, and muscle (PubMed:8402897).
• 细胞定位 [Sterol regulatory element-binding protein 1]: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum. Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed. .; [Processed sterol regulatory element-binding protein 1]: Nucleus .; [Isoform SREBP-1aDelta]: Nucleus .; [Isoform SREBP-1cDelta]: Nucleus .
• 信号通路 Insulin_Receptor;
• 功能 alternative products:Additional isoforms seem to exist,function:Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').,online information:Sterol regulatory element-binding protein entry,PTM:At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.,sequence caution:Intron retention.,similarity:Belongs to the SREBP family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subcellular location:Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.,subunit:Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.,tissue specificity:Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western Blot analysis of 1,mouse-liver 2,hela 3,mouse-brain cells using primary antibody diluted at 1:1000（4°C overnight）. Secondary antibody：Goat Anti-rabbit IgG IRDye 800（ diluted at 1:5000, 25°C, 1 hour）
	Western Blot analysis

产品详情