产品基本信息

• 产品货号 BD-PM6918
• 别名 Vimentin
• 产品名称 Vimentin (8A7) Mouse Monoclonal Antibody
• 类别 抗体产品
• 基因名称 VIM
• 蛋白名称 Vimentin
• 推荐应用 IHC-P,mIHC,ELISA
• 反应种属 Human, Mouse (predicted: Rat)
• 制备方法 The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 7431
• Human Gene Link https://www.ncbi.nlm.nih.gov/gene/?term=VIM
• Human Swissprot No. P08670
• Human Swissprot Link https://www.uniprot.org/uniprotkb/P08670/entry
• 特异性 The antibody can specifically recognize human Vimentin protein. In western blotting of Hela cell lysate, the antibody can label a 54KDa band corresponding to Vimentin.
• 稀释度 IHC-P/mIHC 1:200-400. ELISA 1:500-5000
• 运输及保存条件 -15°C to -25°C/1 year(Do not lower than -25°C)
• 组织表达 Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
• 细胞定位 Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane .
• 功能 function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.,
• 纯化 The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

图片

	Human endometrial adenocarcinoma tissue was stained with Anti-Vimentin Antibody
	Human hepatocellular carcinoma tissue was stained with Anti-Vimentin Antibody
	Human rectal carcinoma tissue was stained with Anti-Vimentin Antibody
	Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue . The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (1:200), Anti-p120 (1:200), Anti-Topoisomerase IIα (1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
	Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue . The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (1:200), Anti-p120 (1:200), Anti-Topoisomerase IIα (1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

产品详情