产品基本信息

• 产品货号 BD-PT7991
• 产品名称 GSDMD n-ternal Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 GSDMD
• 蛋白名称 Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN
• Clonality Polyclonal
• 推荐应用 IF-P,IF-F,IF-ICC,WB,IHC-P
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 制备方法 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• 存储缓冲液 Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
• Human Swissprot No. P57764
• Human Swissprot Link https://www.uniprot.org/uniprotkb/P57764 /entry
• 特异性 The antibody detects endogenous full lenth and n-ternal fragment of gsdmd protein,
• 稀释度 IF-P/IF-F/IF-ICC 1:50-200, WB 1:1000-3000, IHC-P 1:50-300
• 参考分子量 36 55kDa
• 运输及保存条件 Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles
• 组织表达 Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
• 细胞定位 [Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane ; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol .
• 功能 Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
	Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit was diluted at 1:10000
	Immunohistochemical analysis of paraffin-embedded human Moderately differentiated hepatocellular carcinoma Antibody was diluted at 1:200(4° overnight).

产品详情