产品基本信息

• 产品货号 RM4176
• 纯度 Affinity-chromatography
• 别名 c-Myc; Class E basic helix-loop-helix protein 39; MRTL; MYC; Myc proto-oncogene protein; v-myc;
• 产品名称 c-Myc (9U18) Rabbit Monoclonal Antibody
• 类别 抗体产品
• 基因名称 MYC
• 蛋白名称 BHLHE39
• Clonality Monoclonal
• 推荐应用 WB,IHC-P,IF-P,IF-ICC,FCM,IP
• 反应种属 Human,Mouse,Rat
• 存储缓冲液 Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
• Human Gene ID 4609
• Human Swissprot No. P01106
• 免疫原 A synthetic peptide of human c-Myc
• 稀释度 WB 1:1000, IHC-P/IF-P 1:50-1:500, IF-ICC 1:20-1:200, FCM 1:50-1:100, IP 1:20-1:50
• 参考分子量 57-65kDa
• 预测分子量 49kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 IgG
• 注意事项 c-Myc Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• 细胞定位 Nucleus, nucleoplasm. Nucleus, nucleolus
• 功能 Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).

图片

	Western blot detection of c-Myc in HeLa cell lysates using c-Myc antibody(1:1000 diluted).
	Western blot detection of c-Myc in K562,C6,3T3,Hela cell lysates using c-Myc antibody(1:1000 diluted).
	Immunofluorescent analysis of HeLa cells using RM4176 antibody (green), and DAPI (blue).
	Immunofluorescence analysis of HeLa cells labelling c-Myc with RM4176. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with RM4176 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.

产品详情