产品基本信息

• 产品货号 BD-PB4550
• 别名 Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e, CEACAM5, CEA
• 产品名称 CEA Rabbit Polyclonal Antibody (Center)
• 类别 抗体产品
• Clonality Polyclonal
• 推荐应用 WB, FCM, IHC-P-Leica
• 反应种属 Human
• 存储缓冲液 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Human Gene ID NP_004354.2
• Human Swissprot No. P06731
• 特异性 This CEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from the Central region of human CEA.
• 稀释度 WB 1:1000
• 预测分子量 77kDa
• 运输及保存条件 Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• 宿主 Rabbit
• 同种型 IgG
• 背景介绍 CEA is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. It is a receptor for E.coli Dr adhesins.
• 组织表达 Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
• 细胞定位 Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface Note=Localized to the apical glycocalyx surface
• 功能 Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).

图片

All lanes : Anti-CEA Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情